Investigation of Virulence Factors in Microbial Organisms that Associated with Public Health Risk Isolates from Different Environmental Regions

Infectious diseases caused by infected tools in the environments are threaten to safety and public health. Transmission sources of these infectious unknown, but it is thought that non-living materials called fomites, major source acquired infections. Three hundred one swabs were taken from different cultured on blood agar study heamolysis ability isolated bacteria. In this study, MacConkey was used isolate Gram-negative bacteria Sabouraud (SDA) fungi. The biofilm formation test done Congo red plate assay. 41 (13.6%) bacterial isolates obtained (18.27%) fungi (SDA). Staphylococcus aureus more frequent species study. 29% samples showed hemolysin activity 32%of biofilm- producer. Results revealed (7.9%) harbored fimH gene, (9%) icaA Gram-positive 6.3 % fungal had HWP1 gene. Furthermore, (9.3%) total hla gene belong spp. (5.07%) tested possessed hlyA We found our organisms can be transmitted individual another fomites responsible for infection.